hoechst 33258 dapi (Beyotime)

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Beyotime hoechst 33258 dapi
Hoechst 33258 Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 3165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+33258+dapi/Hoechst+33258/pmc12139020-74-113-129
Average 97 stars, based on 3165 article reviews

hoechst 33258 dapi - by Bioz Stars, 2026-09

97/100 stars

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Bioprocessing:

Article Title: Design, optimization, and evaluation of lyophilized lipid nanoparticles for mRNA-based pulmonary mucosal vaccination
Article Snippet: .. Reagent: Sap I restriction endonuclease (New England Biolabs, Beverly, MA, USA), citrate buffer (25 mM, pH 4.0), β-sitosterol, Diethylenetriaminepentaacetic acid (DTPA), mucin (Leagene Biotechnology Co., Ltd., Beijing, PRC), 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000), cholesterol, sucrose (AVT Pharmaceutical Tech Co., Ltd., Shanghai, PRC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), SM-102 (SINOPEG, Xiamen, PRC), Quant-ITTM RiboGreen assay, murine granulocyte‒macrophage colony-stimulating factor (Thermo Fisher Scientific, Waltham, MA, USA), transcription kit, vaccinia capping enzyme, mRNA cap 2′-O-methyltransferase, SARS-CoV-2 Fluc eGFP BA.4/BA.5 pseudovirus (Vazyme Biotech Co. Ltd., Nanjing, PRC), agarose (Biowest, France), mannitol (Roquette Freres, Lestrem, France), PEG6000, nystatin, chlopromazine hydrochloride, amiloride (EIPA), simulants of lung fluid (Solarbio Life Sciences, Beijing, PRC), methyl-β-cyclodextrin (MβCD) (Zibo Qianhui Biological Technology Co., Ltd., Shandong, PRC), lithium chloride, Hoechst 33258 (DAPI), DiI, goat anti-mouse IgG (HRP), goat anti-rabbit IgG (HRP), β-actin rabbit monoclonal antibodies (Beyotime Biotechnology, Shanghai, PRC), CALNPTM RNAi in vitro (Dona Pharmaceuticals Co., Ltd., Beijing, PRC), 50 % yolk emulsion (Haibo Biotechnology Co., Ltd., Qingdao, PRC), goat anti-mouse IgG1 (HRP), goat anti-mouse IgG2a heavy chain (HRP), goat anti-mouse IgA (α-chain specific)-and rabbit monoclonal antibodies against eGFP (Abcam, Cambridge, MA, USA), DMEM, fetal bovine serum (FBS) (HUANKE, Beijing, PRC), RPMI 1640 medium (Wisent Corporation, Nanjing, PRC), Opti-MEM® (Gibco BRL, Grand Island, NY, USA). .. Cell Lines : Mouse bone marrow-derived dendritic cells (DC2.4) (China Center for Type Culture Collection), human renal epithelial cells (293T), mouse monocyte macrophage leukemia cells (RAW264.7) (Shanghai Geneway Biotechnology Co., Ltd.), human lung adenocarcinoma cells (CALU-3) (Wuhan PunoSai Life Science and Technology Co., Ltd.), HEK293-ACE2 overexpressed cells (Novoprotein, Suzhou, China.)

Article Title: Design, optimization, and evaluation of lyophilized lipid nanoparticles for mRNA-based pulmonary mucosal vaccination
Article Snippet: .. Reagent: SapI restriction endonuclease (New England Biolabs, Beverly, MA, USA), citrate buffer (25 mM, pH 4.0), β-sitosterol, Diethylenetriaminepentaacetic acid (DTPA), mucin (Leagene Biotechnology Co., Ltd., Beijing, PRC), 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000), cholesterol, sucrose (AVT Pharmaceutical Tech Co., Ltd., Shanghai, PRC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), SM-102 (SINOPEG, Xiamen, PRC), Quant-ITTM RiboGreen assay, murine granulocyte‒macrophage colonystimulating factor (Thermo Fisher Scientific, Waltham, MA, USA), transcription kit, vaccinia capping enzyme, mRNA cap 2′-O-methyltransferase, SARS-CoV-2 Fluc eGFP BA.4/BA.5 pseudovirus (Vazyme Biotech Co. Ltd., Nanjing, PRC), agarose (Biowest, France), mannitol (Roquette Freres, Lestrem, France), PEG6000, nystatin, chlopromazine hydrochloride, amiloride (EIPA), simulants of lung fluid (Solarbio Life Sciences, Beijing, PRC), methyl-β-cyclodextrin (MβCD) (Zibo Qianhui Biological Technology Co., Ltd., Shandong, PRC), lithium chloride, Hoechst 33258 (DAPI), DiI, goat anti-mouse IgG (HRP), goat anti-rabbit IgG (HRP), β-actin rabbit monoclonal antibodies (Beyotime Biotechnology, Shanghai, PRC), CALNPTM RNAi in vitro (Dona Pharmaceuticals Co., Ltd., Beijing, PRC), 50 % yolk emulsion (Haibo Biotechnology Co., Ltd., Qingdao, PRC), goat anti-mouse IgG1 (HRP), goat anti-mouse IgG2a heavy chain (HRP), goat anti-mouse IgA (α-chain specific)-and rabbit monoclonal antibodies against eGFP (Abcam, Cambridge, MA, USA), DMEM, fetal bovine serum (FBS) (HUANKE, Beijing, PRC), RPMI 1640 medium (Wisent Corporation, Nanjing, PRC), Opti-MEM® (Gibco BRL, Grand Island, NY, USA). .. Cell Lines: Mouse bone marrow-derived dendritic cells (DC2.4) (China Center for Type Culture Collection), human renal epithelial cells (293T), mouse monocyte macrophage leukemia cells (RAW264.7) (Shanghai Geneway Biotechnology Co., Ltd.), human lung adenocarcinoma cells (CALU-3) (Wuhan PunoSai Life Science and Technology Co., Ltd.), HEK293-ACE2 overexpressed cells (Novoprotein, Suzhou, China.)

In Vitro:

Article Title: Design, optimization, and evaluation of lyophilized lipid nanoparticles for mRNA-based pulmonary mucosal vaccination
Article Snippet: .. Reagent: SapI restriction endonuclease (New England Biolabs, Beverly, MA, USA), citrate buffer (25 mM, pH 4.0), β-sitosterol, Diethylenetriaminepentaacetic acid (DTPA), mucin (Leagene Biotechnology Co., Ltd., Beijing, PRC), 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000), cholesterol, sucrose (AVT Pharmaceutical Tech Co., Ltd., Shanghai, PRC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), SM-102 (SINOPEG, Xiamen, PRC), Quant-ITTM RiboGreen assay, murine granulocyte‒macrophage colonystimulating factor (Thermo Fisher Scientific, Waltham, MA, USA), transcription kit, vaccinia capping enzyme, mRNA cap 2′-O-methyltransferase, SARS-CoV-2 Fluc eGFP BA.4/BA.5 pseudovirus (Vazyme Biotech Co. Ltd., Nanjing, PRC), agarose (Biowest, France), mannitol (Roquette Freres, Lestrem, France), PEG6000, nystatin, chlopromazine hydrochloride, amiloride (EIPA), simulants of lung fluid (Solarbio Life Sciences, Beijing, PRC), methyl-β-cyclodextrin (MβCD) (Zibo Qianhui Biological Technology Co., Ltd., Shandong, PRC), lithium chloride, Hoechst 33258 (DAPI), DiI, goat anti-mouse IgG (HRP), goat anti-rabbit IgG (HRP), β-actin rabbit monoclonal antibodies (Beyotime Biotechnology, Shanghai, PRC), CALNPTM RNAi in vitro (Dona Pharmaceuticals Co., Ltd., Beijing, PRC), 50 % yolk emulsion (Haibo Biotechnology Co., Ltd., Qingdao, PRC), goat anti-mouse IgG1 (HRP), goat anti-mouse IgG2a heavy chain (HRP), goat anti-mouse IgA (α-chain specific)-and rabbit monoclonal antibodies against eGFP (Abcam, Cambridge, MA, USA), DMEM, fetal bovine serum (FBS) (HUANKE, Beijing, PRC), RPMI 1640 medium (Wisent Corporation, Nanjing, PRC), Opti-MEM® (Gibco BRL, Grand Island, NY, USA). .. Cell Lines: Mouse bone marrow-derived dendritic cells (DC2.4) (China Center for Type Culture Collection), human renal epithelial cells (293T), mouse monocyte macrophage leukemia cells (RAW264.7) (Shanghai Geneway Biotechnology Co., Ltd.), human lung adenocarcinoma cells (CALU-3) (Wuhan PunoSai Life Science and Technology Co., Ltd.), HEK293-ACE2 overexpressed cells (Novoprotein, Suzhou, China.)

Emulsion:

Article Title: Design, optimization, and evaluation of lyophilized lipid nanoparticles for mRNA-based pulmonary mucosal vaccination
Article Snippet: .. Reagent: SapI restriction endonuclease (New England Biolabs, Beverly, MA, USA), citrate buffer (25 mM, pH 4.0), β-sitosterol, Diethylenetriaminepentaacetic acid (DTPA), mucin (Leagene Biotechnology Co., Ltd., Beijing, PRC), 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2000), cholesterol, sucrose (AVT Pharmaceutical Tech Co., Ltd., Shanghai, PRC), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), SM-102 (SINOPEG, Xiamen, PRC), Quant-ITTM RiboGreen assay, murine granulocyte‒macrophage colonystimulating factor (Thermo Fisher Scientific, Waltham, MA, USA), transcription kit, vaccinia capping enzyme, mRNA cap 2′-O-methyltransferase, SARS-CoV-2 Fluc eGFP BA.4/BA.5 pseudovirus (Vazyme Biotech Co. Ltd., Nanjing, PRC), agarose (Biowest, France), mannitol (Roquette Freres, Lestrem, France), PEG6000, nystatin, chlopromazine hydrochloride, amiloride (EIPA), simulants of lung fluid (Solarbio Life Sciences, Beijing, PRC), methyl-β-cyclodextrin (MβCD) (Zibo Qianhui Biological Technology Co., Ltd., Shandong, PRC), lithium chloride, Hoechst 33258 (DAPI), DiI, goat anti-mouse IgG (HRP), goat anti-rabbit IgG (HRP), β-actin rabbit monoclonal antibodies (Beyotime Biotechnology, Shanghai, PRC), CALNPTM RNAi in vitro (Dona Pharmaceuticals Co., Ltd., Beijing, PRC), 50 % yolk emulsion (Haibo Biotechnology Co., Ltd., Qingdao, PRC), goat anti-mouse IgG1 (HRP), goat anti-mouse IgG2a heavy chain (HRP), goat anti-mouse IgA (α-chain specific)-and rabbit monoclonal antibodies against eGFP (Abcam, Cambridge, MA, USA), DMEM, fetal bovine serum (FBS) (HUANKE, Beijing, PRC), RPMI 1640 medium (Wisent Corporation, Nanjing, PRC), Opti-MEM® (Gibco BRL, Grand Island, NY, USA). .. Cell Lines: Mouse bone marrow-derived dendritic cells (DC2.4) (China Center for Type Culture Collection), human renal epithelial cells (293T), mouse monocyte macrophage leukemia cells (RAW264.7) (Shanghai Geneway Biotechnology Co., Ltd.), human lung adenocarcinoma cells (CALU-3) (Wuhan PunoSai Life Science and Technology Co., Ltd.), HEK293-ACE2 overexpressed cells (Novoprotein, Suzhou, China.)

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hoechst 33258 dapi (Beyotime)

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